- pieces of the leaf were immediately cut using a hole punch with a diameter of 6 mm.
在叶片表面切割一个直径6毫米孔大小的部分。 - The pieces were ground in a clear mortar with a pestle, and then the leaf pigment mixture was
placed in a 25‐ml volumetric flask with 95% ethanol.
将其放入研钵中进行研磨，然后将混合的色素倒入装有25ml浓度为95%酒精的烧瓶中。
All experiments were performed in a dark room.
上述操作在黑暗环境中进行。 - Subsequently, we placed a plastic tube filled with a mix of the solution and chlorophyll in a centrifuge, which has a rotational speed of 3,200 r/min, for 10 min of centrifugation.
随后我们将混合有溶液和叶绿素的塑料试管放入离心机中进行10min离心分离操作。离心机转速为每分钟3200转。 - The absorbance of the supernatant was measured using a Lambda 900 spectrophotometer (Perkin‐Elmer, Waltham, MA, USA).
上层浮液的吸光度由Lambda900分光光度计测定。 - Based on the method provided by Wintermans and De Mots (1965), LCC (μg/cm2) was calculated.
基于W.和D.于1965年提出的方法，计算叶片叶绿素含量（LCC）。
Wintermans, J. F. G. M., & De Mots, A. (1965). Spectrophotometric characteristics of chlorophylls a and b and their phenophytins in ethanol. Biochimica et Biophysica Acta (BBA)—Biophysics including Photosynthesis, 109, 448–453. https://doi.org/10.1016/0926‐6585(65)90170‐6.
Li W, Sun Z, Lu S, Omasa K. Estimation of the leaf chlorophyll content using multiangular spectral reflectance factor. Plant Cell Environ. 2019;1–14. https://doi.org/10.1111/pce.13605.